First of its kind molecular probe to identify and detect exosomes
ELAEXIA® - Elective Labelling of Exosomes for Identification and Analysis
- Designed for detection of exosomes by flow cytometry and stringently developed to generate a specific, clear signal without false-positive background
- Precise and specific binding to exosome bilayer membrane
- Excludes lipoproteins
- Novel technology, independent of highly variable protein markers which identify exosome subpopulations
- Direct labelling of exosomes in cell culture supernatant and plasma
- Compatible with antibody co-staining and multiparametric analysis
- Fast and Easy-to-use labelling protocol
ELAEXIA® FITC can be used for the following applications:
- Exosome labelling in cell culture supernatant and plasma for multiparametric flow cytometry analysis
- Labelling of exosomes for immunofluorescence microscopy and nano imager analysis
- ELAEXIA® labelling for specific isolation by Fluorescence Activated CellSorting (FACS) of exosomes for further protein analysis such as Westernblot and mass spectrometry (MS)
- ELAEXIA® labelling to monitor and control exosome purification and manufacturing
- Labelling of exosomes for Nanoparticle Tracking Analysis (NTA)
Supportive Data
Figure
Left panel: Exosome detected in th cell culture supernatant of breast cancer cells SKBR-3 with ELAEXIA®-FITC by mans if flow cytometry. In the right upper quadrant double positive exosomes are shown whic are ELEAXIA and CD9 positive.
Right panel: Negative control – Treatment with 1% Triton solubilizes the exosomes
-> no vesicles can be detected
Figure
Nanoparticle TrackingAalysis (NTA) of exosomes from plasma sorted by flow cytometry and ELAEXIA®-FITC. ELAEXIA© exosomes have the size of 30 to 200 nm.
ELAEXIA® is a registered trademark of therawis diagnostics GmbH
