The ELAEXIA®-T MS kit enriches exosomes from biofluids such as cell culture supernatant or human plasma. The enrichment is performed by positive selection by means of flow cytometry sorting applying ELAEXIA®-T MS which binds specifically with high affinity to the lipid membrane of exosomes.
- Catalog no.: ELX-100-01
- List price: upon request
- Product name: ELAEXIA®-T MS
- Size: 10 isolations
- Quantity: 200µl
For research use only!
Instruction for Use
Safety Data Sheet
Background
Extracellular vesicles (EVs) are generated almost by any cell and different subcellular compartments and, therefore, EVs display a significant heterogeneity and diversity. Current applied methods for exosome purification are ultracentrifugation, precipitation incl. PEG, immunocapture with antibodies against CD9, CD63, CD81, TSG, Rab5, HLA-ABC, microfluidic isolation, filtration, and size exclusion chromatography. However, filtration, size exclusion chromatography or precipitation isolate a mixture of microvesicles, lipoproteins and exosomes and these methods also bear the risk of damage or destruction of exosomes. CD9, CD81, CD63 immunocapturing to purify microvesicles and exosomes will isolate subpopulations of exosomes because the extracellular versicles are loaded with different protein cargo depending on the state of the cells they originate from. The lack of standardization and reproducibility hamper the verification and validation required for In Vitro Diagnostic workflows.
Our novel, highly selective method is based on the exosome biogenesis. Exosome biogenesis occurs in cells and at the molecular level inside the Multi-Vesicular Body (MVB) compartment. The donor membrane for the budding vesicle is the MVB membrane (Subra et al., 2007). Lipids such as phosphatidylserine and phosphatidylethanolamine, usually in the inner leaflet of the membrane of cells, form through this inward budding of MVBs the outer leaflet of the exosome membrane. ELAEXIA®, Elective Labelling of Exosomes for Identification and Analysis, binds specifically to phosphatidylethanolamine in the exosome lipid bilayer membrane.
Data and images
Mass spectrometry analysis of exosome enrichment by ELAEXIA®-T MS Exosome kit
Fig. 2 Cryo-electron microscopy analysis of exosomes isolated by flow cytometry sorting applying ELAEXIA®-T MS from human plasma demonstrated in all vesicles the characteristic lipid bi-layer membranes. Vesicles with single lipid membrane typically for lipoproteins were not detected providing evidence that ELAEXIA® isolated exosomes are lipoprotein free.
